Journal: Journal of Neuropathology and Experimental Neurology
Article Title: Inhibiting Matrix Metalloproteinases Protects Evoked Electromyography Amplitudes and Muscle Tension in the Orbicularis Oris Muscle in a Rat Model of Facial Nerve Injury
doi: 10.1093/jnen/nlac041
Figure Lengend Snippet: (A1–A6, B1–B6, C1–C6) Immunostaining for MMP3 of the orbicularis oris in control and prinomastat groups on days 3, 7, and 14 after facial nerve injury. (D) Western blot for MMP1, MMP2, MMP3, MMP7, and MMP9 in the orbicularis oris in prinomastat and control groups. MMP1, MMP2, MMP3, MMP7, and MMP9 expression levels in control and prinomastat groups at days 0, 3, 7, and 14 after facial nerve injury. (E) Bar chart of MMP1, MMP2, MMP3, MMP7, and MMP9 at days 0, 3, 7, and 14 after facial nerve injury in the prinomastat group. (F) Bar chart of MMP1, MMP2, MMP3, MMP7, and MMP9 at days 0, 3, 7, and 14 after facial nerve injury in the control group. The error bars represent SD; scale bar: 20 μm.
Article Snippet: The following antibodies and reagents were used: Goat antiagrin antibody (AF550, R&D Systems, Minneapolis, MN), rabbit polyclonal anti-MMP1 (ab51074, Abcam, Cambridge, UK), rabbit polyclonal anti-MMP2 (ab92536, Abcam), rabbit polyclonal anti-MMP3 (ab53015, Abcam), rabbit polyclonal anti-MMP7 (PA5-79684, Invitrogen, Waltham, MA), rabbit monoclonal anti-MMP9 (ab76003, Abcam), rabbit polyclonal anti-GAPDH (ab9485, Abcam), MMP3 inhibitor prinomastat (HY-12170A, MedChemExpress, Princeton, NJ), phospho-tyrosine mouse mAb (P-Tyr-100, Cell Signaling Technology, Danvers, MA), rabbit polyclonal anti-MuSK (GTX25619, GenTex, Zeeland, MI), AlexaFluor α-bungarotoxin (B13422, Life Technologies, Carlsbad, CA), AlexaFluor594-labeled donkey antigoat secondary antibody (A11058, Invitrogen) AlexaFluor594 donkey antirabbit (A21207, Invitrogen), and AlexaFluor488 α-bungarotoxin (B13422, Life Technologies).
Techniques: Immunostaining, Western Blot, Expressing